The two different data import options are tailored to quickly get your data into SoftMax Pro to provide a single source of software for all your data reduction needs. With optimized reagents, option to operate the. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. Clone Screening. The instrument settings were identical to those used in Example 1 above. 1. Choose the desired plate type, using the upper dropdownThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. This offer includes SpectraMax i3x, SpectraMax. 1. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. Table 2 summarizes the results for the uniformity test. Company Info. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Please contact your Molecular Devices representative for configuration options. Plate Formats: 6 to 384-well microplates. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular. For more sample throughput, both readers can be easily. Microplate Stacker and Washer. Cells were thawed and propagated according to the recommended method specified by the supplier. Longside barcode reader: 0310-5101. 9. Three low volume 24-well bottom slides. (Figure 1). 0200-6262. Wait 20 seconds. With various on-board features and. 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. It consists of a SpectraMax® Microplate Reader, Peak Robotics S-LAB Plate Handler with the S-LAB software, and the AquaMax® 4000 Microplate Washer. With optimized reagents, option to operate the. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. book Page 1 Tuesday, July 6, 2010 2:08 PM Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 635. The Back Panel Figure 1. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 1. 5. 1 sec. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial Rack Bottom Label 2D Reader) Microscan: MS-3 Laser Bar Code Reader: Thermo Fisher Scientific: VisionMate 96 (Vial Rack Bottom Label 2D Reader). We therefore gathered salient. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. sensitivity or performance. visible absorbance, fluorescence. Videos & Webinars. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. The plate reader optics are tunable for absorbance. 3860 N First Street San Jose, CA 95134. Perform the following steps to install the drivers: Start > Control Panel > System. If no sound is heard during initialization, contact technical support. W×D×H. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader. Microplate Reader Comparison Charts Published Dec 16, 2020 | Updated Dec 16, 2020 Products : General, FilterMax F5 reader Please click on the links below to. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. We would like to show you a description here but the site won’t allow us. Recombinant protein expression Nucleocapsid. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. 1700 +1 800. In the M2, M5 and Plus. Fill a clean plate with water. First, as mentioned before, the walkaway time significantly. the iD5 reader’s filter system. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices Supplier: Molecular Devices Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength. It will operate in 96 and 384 well plate format, and has installed filters for fluorescence of 370, 485 and 535 nm for excitation. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 0. For cryo preservation cells were scaled up in 10-layer factories (Corning Cat. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Plate reader. Clone Screening. The Versamax Microplate Reader is built around a grating monochromator. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. The presence of VCAM-1 indicates a positive inflammatory response. 001 EU/mL) was added in order to test the sensitivity of the SpectraMax i3 reader. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. 0077 OD 0. 9. M5. V. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Select Start>Control panel>Device Manager. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. 1). Fixed cells stained with fluoresceinconjugated anti-VCAM are identified with the Marker Expression Protocol. PURPOSE. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesMolecular Devices Software. Select Update Driver. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. The guide also covers the software features, the specifications, and the accessories of the systems. The whole plate can be detect in a single run with ease and flexible. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. 0. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Supersedes 1. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. Molecular Devices. Published Dec 16, 2020 | Updated Dec 16, 2020. 1 software Download page Molecular Devices Software SoftMax® Pro. Absorbance Plate Reader Comparison Chart. 8×15×8. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. The adapter is required for optimum performance with standard 96-well and 384-. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. With optimized reagents, option to operate the readers. Molecular Devices’ SpectraDrop TM MicroVolume Microplate for SpectraMax ® microplate readers offers higher throughput than any other low-volume microplate, with up to 64 samples per plate. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 9. Molecular Devices offers a broad range of technologies for high-throughput screening, genomic and cellular analysis, colony selection, and microplate detection. 6") Weight. Multimode microplate readers offer flexibility, and ease of use. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. The test compound menadione was added to cells in a 1:3. Lastly, we demonstrate the utility of this novel fluorescence-based assay to evaluate the activities of. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Introduction. 1. 2. 1-2 sec. PathCheck sensor corrects optical pathlength & pipetting variation. SpectraTest® Validation Package User Manual Download Page. Purchase is subject to Molecular Devices standard terms and conditions of sale. M5. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Double-click the unknown device with the yellow warning icon. The cuvette must be in place when you read the plate. Supplier: Molecular Devices. Calcium ions (Ca2+) play a key role in cellular homeostasis involving calcium channel and GPCR function, which plays a critical role in many disease pathologies. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. What are the Vmax plate reader specifications? Recommended Articles SoftMax® Pro 7. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Set up Identify Acquire Analyze Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. The reader measures both flat and round 96-well microplates and. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. For more sample throughput, the system can be easily integrated into a full roboticOnly Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. This can increase throughput and efficiency, thus saving time and space in the lab. Plate stacker and robot integration The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within minutes and begin reading microplates with seven mouse clicks. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Category 2 and 3 microplate readers use gradient heating as well. Luminescence is the emission of light by a substance as a result of a chemical reaction (chemiluminescence) or an enzymatic reaction (bioluminescence). From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. Instrument settings can be saved as a protocol file. FlexStation 3 reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. Read height should be optimized forVmax® plate reader specifications. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. Detection cartridge modularity allows for easy addition of new read modes within minutes without. For biochemical assays, the washer removes unbound material and unreacted reagents. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). SpectraMax® Microplate Readers and SoftMax® Pro Software. 005 EU/mL to 5. Plate chamber design of the SpectraMax category 1 microplate readers. 1-1. Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. With The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. “Sensitivity is pretty much clutch for luminescence,” says Daly. D. Figure 1. ” So, acquisition and analysis come from one software system. View SoftMax Pro GxP. S. These tools can be used in conjunction with SoftMax® Pro Software validation package and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance. Customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. A control well was set up to verify the accuracy of the standard curve. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Title: F3/F5 Multi Mode Microplate Readers | Molecular Devices Author: Molecular Devices, LLC Subject: Learn how the F3/F5 Multi-mode microplate readers can utilize its filter sets to accurately perform measurements with up to five modes of detection. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Evaluation of BioTek's Synergy H4 Plate Reader for Fluorescent Detection. A). The absorbance (A405) can be measured by the plate reader and is proportional to the endotoxin concentration in the sample. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. Integrated to. 312 gain calibration failed. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Plate Reader-2 features a wide range of high-performance multi-mode reader capabilities ideal for life science research on the International Space Station (ISS. When connecting for the first time to either the SpectraMax L reader or StakMax system. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Molecular Devices’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. Important things to consider when purchasing a multimode microplate reader. Turn the reader off by pulling the power cord. Microplate Readers. US HEADQUARTERS. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Our microplate readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. 1. Compact designs. Ensure data accuracy of your reader. Lift up the lid over the chamber to insert or remove a cuvette. 2. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. To update the driver. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 0 . A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. FlexIII. Increase productivity with patented microplate reader technologies. Used. 340 PC384. Select Start>Control panel>Device Manager. ISM Equipment Classification (Group 1, Class A) This equipment is. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in writing. Turn the reader on and secure the USB cable. The computer controlling the plate reader runs Windows XP. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. . SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. Published Dec 16, 2020 | Updated May 19, 2023. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. M2. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Monday to Friday 8am - 5pm GMT. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. 00 USD. Upgradeable modules are also available. 100 μL of blank or endotoxin standard (in. 96-well plate and 12,500-20,000. 7°C (gradient heating design). Description Figure 1. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. This document is provided to customers who have purchased Molecular Devices, Inc. Laboratory automation workflows can help. Number of Citations*: 8350. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Explore our fully-integrated,. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. For nearly 30 years, Molecular Devices has provided scientists with . Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Recertification service. 2. Absorbance, fluorescence, and luminescence detection with upgradeable high-performance capabilities. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The lower limit of detection (LLD) was defined as the level of. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 5: Schematic of. 1. This document is copyright protected and any reproduction of this document, in whole or. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. It may be named "Pothos". The plate reader optics are tunable for absorbance, fluorescence intensity,. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. BMG Labtech - THERMOstar. , attenuation mode = out. Next generation SpectraMax® absorbance microplate readers. Absorbance Plate Reader Comparison Chart. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 0A Sunnyvale, CA Made in USAFilter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 0200-6263. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. Create custom fluorescence assays without having to purchase expensive filter sets. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. These systems are hybrids that allow you to choose between. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. 0 × 103 to 2. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Since cells are nearly confluent, the protocol. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The FLIPRTETRA, which debuts today at. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Tristar 3 Multimode Microplate Reader. SpectraMax Absorbance, Fluorescence. The adapter is required for optimum performa nce with standard 96-well and 384-. The adapter is required for optimum performance with standard 96-well and 384-. United States Phone: 800-635-5577 408-747-1700. We would like to show you a description here but the site won’t allow us. Molecular Devices SpectraMax® microplate readers support a multitude of assays for nucleic acids and protein detection. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. The adapter is required for optimum performance with standard 96-well and 384-. Robust, high value microplate reader designed to deliver research-grade results to any laboratory. Patented pathlength correction for better absorbance accuracy Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. 305 reference level saturation. An industry-renowned instrument for monitoring GPCRs and ion channels. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Register to download your free eBook today. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax:. Reader Documentation Version No. 1. SoftMax® Pro 7. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Pre-defined protocols. Sunnyvale, Calif. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Please click on the links below to view the Microplate Reader Comparison Tables. Eight filter options provide simplified assay setup. If it looks fine, then proceed. The advantage of using labeled probes is detection specificity. Integrated stacker option. ®100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 72 MB. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 4: Test tube cover. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. 5: Schematic of the back panel of a reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Timothy Bolus, MPM, MBA, Compliance Program Manager. In . #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. 0 OD for earlier models. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Note: removal of medium, followed by addition of a 1X solution of calcein AM and EthD-III, is. 00. #iD3) Methods. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. 0) OD (0. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Step 1: Turn on your microplate reader. A control well was set up to verify the accuracy of the standard curve. We would like to show you a description here but the site won’t allow us. DOCUMENTATION. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Published Dec 16, 2020 | Updated Dec 16, 2020. Supplier: Molecular Devices. 0. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Check software compatibility with Windows 10 and locate your software installer. One plate carrier adapter is provided with the instrument. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. Plate Reader. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The EMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. The ultimate guide to microplate readers is an eBook that tells you everything you need to know. PURPOSE. 6 % of reading 4. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Easy-to-use touchscreen allows you to quickly set up your experiments, use preconfigured analysis methods, and view tutorial videos. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Gregory et al. The instrument will read theThe FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A)Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. The plate is set up for reading and images are acquired according to specified parameters. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and. please click here. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. Endpoint. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Page 1 of 8 . 4 °C above ambient to 45 °C. Microplate Readers. This process should take about 3 - 5 minutes. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell. Restart the system. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Search. On the back, in the right hand top corner, there. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Detection cartridge modularity allows for easy addition of new read modes within minutes without.